详细信息

葡萄糖检测试剂 glucose assay reagent 20 mL
淀粉检测试剂 starch assay reagent 1 小瓶
淀粉检测标准品 starch assay standard 5 g

Description
Application This kit is for the quantitative, enzymatic determination of native starch in food and other materials. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.



Properties
usage sufficient for 20 assays
storage temp. 2-8°C



Safety
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36/37/39

References
reference Beutler, H. Meth. Enzym. Anal. 3rd ed., 6, 2-10, (1984)

Printable Reviews

Components
Standard component